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In the case of a specific DNA fragmentation, the order of fragments in DNA can be learned mature ladies Flint this analysis, nondestructively and quickly, without fractionating the fragments and other methods of fragmentation.

If the DNA nucleotide sequence is known except for some sites and uncertain portions, the analysis determines these sites and the accuracy of the sequence at the portions. This information may complement exact methods of DNA sequencing.

The proposed analysis is applied to bacteriophage phiX, whose melting curve is known. The results are compared to and found to be in an excellent agreement with the known phiX nucleotide sequence. National Center for Biotechnology InformationU.

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This article has been cited by other articles in PMC. Sanger F, Coulson AR.

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J Mol Biol. DNA sequencing with chain-terminating inhibitors. A new method for sequencing DNA. Nucleotide sequence of the gene coding for the bacteriophage MS2 coat protein.

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A-protein gene of bacteriophage MS2. Nucleotide jy of bacteriophage phi X DNA. The genome of simian virus Melting fine structure of DNA fragments of known base sequence from theta X The inverse problem for DNA.

Biochem Biophys Res Commun. Fine structures in denaturation curves of bacteriophage lambda DNA. Their relation to the intramolecular heterogeneity in base compositon.

Biochim Biophys Acta. High resolution thermal denaturation of DNA: High-resolution thermal denaturation of DNA. Theoretical and practical considerations for the resolution of thermal subtransitions.

Hyperfine structure in melting profile of bacteriophage lambda DNA. Long range homogeneity of physical stability in double-stranded DNA. Location of the cooperative melting regions in bacteriophage fd DNA.

Recursion relation lookking of probability profiles for specific-sequence macromolecules with long-range correlations. Spectral analysis of high resolution direct-derivative melting curves of DNA for instantaneous and total base composition.

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Spectral analysis of the intramolecular heterogeneity of lambda-DNA. Determination of DNA composition and concentration by spectral analysis. Associated Data Supplementary Materials.

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